Let's say if I have to find "length per KB" of a read.
How should I go about it ?
I can find the "length" by subtracting the END coordinate with the read with the START coordinate.
Let's say Read 1 spans from 300 to 330. So, it's length is 30.
Does "Length per KB" mean I divide 30 by 1000 i.e 0.030 ?
JC, thanks a lot ! RPKM is exactly what I am trying to understand. :)
the original concept applied to RNAseq is described in http://www.nature.com/nmeth/journal/v5/n7/full/nmeth.1226.html