Extracting Specific Reads From Vcf File

Question

[Bnfoguy]

Hello,

I am trying to extract indels with coverage ~1 and they are in a VCF file. How do I write a python script or a shell command to extract those indels with coverage 1 only?

This is the data:

chr1    1548545 .       T       TAGATCG 22.5    .       INDEL;DP=2;VDB=0.0251;AF1=1;AC1=2;DP4=0,0,1,0;MQ=60;FQ=-37.5    GT:PL:GQ        1/1:60,3,0:6
chr1    1573532 .       C       CT      4.42    .       INDEL;DP=1;AF1=1;AC1=2;DP4=0,0,0,1;MQ=44;FQ=-37.5       GT:PL:GQ        0/1:40,3,0:3
chr1    1577221 .       AAC     AACAAAC 81.2    .       INDEL;DP=2;VDB=0.0201;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-40.5    GT:PL:GQ        1/1:120,6,0:10
chr1    1577223 .       C       CAAAC   81.2    .       INDEL;DP=2;VDB=0.0185;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-40.5    GT:PL:GQ        1/1:120,6,0:10
chr1    1584063 .       T       TT      3.25    .       INDEL;DP=1;AF1=1;AC1=2;DP4=0,0,1,0;MQ=60;FQ=-37.5       GT:PL:GQ        0/1:38,3,0:4
chr1    1635699 .       AA      A       3.66    .       INDEL;DP=2;VDB=0.0231;AF1=1;AC1=2;DP4=0,0,1,1;MQ=20;FQ=-40.5    GT:PL:GQ        1/1:40,6,0:4
chr1    1637760 .       T       TTTCTTT 22.5    .       INDEL;DP=1;AF1=1;AC1=2;DP4=0,0,0,1;MQ=60;FQ=-37.5       GT:PL:GQ        1/1:60,3,0:6

Thank you for your help!

Answer 1

[Pierre Lindenbaum]

something like

awk -F '      '       '(!($4 ~ /[ATGC]/ && $5 ~/[ATGC]/ ))' < your.vcf | grep "DP=1;"

or better

grep INDEL < your.vcf | grep "DP=1;"

[Bnfoguy]

Thanks Mr. Lindenbaum. You are a lifesaver!

Answer 2

[tommy.carstensen]

Alternatively: awk '/INDEL/ && /DP=1/' your.vcf

Answer 3

[bioinfo]

I prefer ( I assume the vcf file contains just Indels not SNPs)

more indel.vcf | grep "DP=1" &gt > filtered.indel.vcf

If you have SNPs and indel together in the same vcf file then

more indel.vcf | grep "INDEL" | grep "DP=1" &gt > filtered.indel.vcf

Answer 4

[Begali]

hi

I am new for tools such as samtools, bcftools, BWA and GATK and I am working on RADSeq I reach for point call variants which include ( INDEL and SNP) now time to filter them I could not do that by GATK with VQSR which reuired resources bundle folder which are not available for my organisms plant ( 5 samples for Cardamine hirsuta and 197 samples for Arabidopsis thaliana ) so only can do Hard-filtering however I need to plot my data to see my original distribution so I can determine thresholds which need to be filtered by considering lower est thresholds ... so I need to grep only INFO col first then how to select DP ,VDB ;AF1;MQ and so one so I can obtain file for each one which will be easier to plot then in R ...

Thanks in advance