How To Compare Methylated Genes Across Developmental Stages

Question

[Mandar]

I have a list of many methylated genes at different developemental stages what kind of different bioinformatics analysis i can carry out with those genes??

Thanks in advance.

[Arun]

The most obvious thing that comes to my mind is to check if the amount of methylation is different across developmental stages for any/all those genes.

[Mandar]

Thank you arun but that analysis has been done.can you suggest any other analysis which is specifically carry out for the methylated genes?

Answer 1

[Gjain]

have a look at this paper:

Developmental features of DNA methylation during activation of the embryonic zebrafish genome

Zygotic genome activation (ZGA) occurs at the mid-blastula transition (MBT) in zebrafish and is a period of extensive chromatin remodeling. Genome-scale gametic demethylation and remethylation occurs after fertilization, during blastula stages, but how ZGA relates to promoter DNA methylation states is unknown. Using methylated DNA immunoprecipitation coupled to high-density microarray hybridization, we characterize genome-wide promoter DNA methylation dynamics before, during and after ZGA onset, in relation to changes in post-translational histone modifications and gene expression.

I hope this is useful.

Answer 2

[Mandar]

No,i didnt find it so much relevant,can anybody has others idea?