Hi all,
I'm quite new to flow cytometry, since i have mostly perfomed transcritionnal analyses before. I discovered the flowCore, flowViz, flowXXX packages in bioconductor and found it amazing.
I have a series of data with two different panels (tubes) for each patient. As there are some common markers in both panels, i was wondering if i could merge the two into a single flowSet with more channels.
In details :
- for panel A I have SSC, FSC, CD45, CD3, CD4, CD8, HLA-DR
- for panel B I have SSC, FSC, CD45, CD3, CD5, CD19, CD16
I would like to obtain a single merged-panel with : SSC, FSC, CD3, CD45, CD4, CD8, CD5, CD19, CD16, HLA-DR
I was thinking that the dataset is composed roughly of a matrix with for each leukocyte (event, =row) a value for all channels above (columns). So, maybe it would be possible to try to clusterise by pairs the rows of panel A and those of panel B on common values of SSC, FSC, CD45 and CD3. The result dataset would take the mean of the common channels and then the single values for CD4, CD5, CD8, CD16, CD19, HLA-DR.
I would be glad to have your comment on this before starting. Maybe it has already been done, or maybe one would have a comment to take into accoutn before i start ?
Thanks in advance.
