Chip-Exo Illumina Data Sets?
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12.1 years ago

Does anybody know of any publicly available ChIP-exo datasets done with Illumina sequencing?
This paper seems to be a recent reference to the technique:
http://www.ncbi.nlm.nih.gov/pubmed/22153082

But they did SOLiD sequencing, I am looking for Illumina sequencing of ChIP-exo to test it through a method I am developing.

Any ideas?

chip-seq illumina • 3.5k views
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As far I know, the Pugh lab is the only group that's published ChIP-exo data so far. They seem attached to SOLiD, so no dice on Illumina data. A cursory search of the SRA supports this, though I'd be happy to be proven wrong:

http://sra.dnanexus.com/?result_type=Study&show=&q=chip-exo

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Yes, these two are also Chip-exo from SOLiD, it seems it's tricky to get it to work with Illumina NGS.

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Have you tried converting the reads to sequence space and working with that?

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Do you think that would turn the reads into Illumina-like?

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Besides machine and assay biases, as far as i know SOLID sequencing provides an error correction code for the reads, i don't see a problem with just converting them to sequence space. You may need to scale quality but i believe the converting tool would take care of that.

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11.2 years ago
UnivStudent ▴ 440

New article this week in Nature from the Pugh lab: Genomic organization of human transcription initiation complexes. It uses HiSeq data this time instead of SOLiD as in their first paper. SRA Accession: SRA067908.

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