Orthomcl Blast Error: No Hits Are Being Saved

Question

[User 6777]

when i try to run blast in orthomcl using the command below:

blastp -query goodProteins.fasta -db goodProteins.fasta -evalue 1e-5 -out goodProteins.blast -outfmt 6 -seg yes -soft_masking true -max_target_seqs 999999999999 -num_threads 8

it returns

BLAST query/options error: No hits are being saved

although i repeatedly checked that my previous commands are alright;;

Plz help... thanks in advance..

Answer 1

[Manu Prestat]

I guess the db should be formated as a "blast db" first. Look a the makeblastdb utility:

makeblastdb -help