From Where To Start Next-Gen-Sequencing?

Question

[Thaman]

Hi,

I have been hearing so much about Next Generation Sequencing (NGS) recently and going to take short-intensive course on the January. But, I want to know atleast something about it in advance. What are the things I should I know to start NGS? I am beginner in Bioinformatics and trying to learn so many things at the same time. What are good way of starting NGS and different packages related to it? What are the resources I have to go through? I have google and there are so much about it but I dont to from where to start as I am beginner in these things. I know this field taking so much height and it's important for Bioinformatician. What are basic things I have to learn about NGS so within two months I will be able to talk about it freely?

Can you give me guideline so I can give good start in NGS?

Thank you

Answer 1

[Rm]

I Am Preparing A Course On Ngs: Any Suggestion ?
for real data use ncbi SRA

Answer 2

[Mary]

I just remembered this page of Bioinformatics--there was a special NGS collection of articles they put together.

http://www.oxfordjournals.org/our_journals/bioinformatics/nextgenerationsequencing.html

You can see the dates vary, but sometimes there will be some good background in the older articles. But even the category breakdown may be helpful for you to understand the different aspects of the issues.

Answer 3

[Alastair Kerr]

also see this previous thread

[SES]

Link is broken. Perhaps it should point to Comprehensive Intro To Next Generation Sequencing?

[Ram]

I've fixed it now - a half decade too late, I know.

Answer 4

[Philippe]

Well, I think a good approach would be to select a publication who used a standard technique similar to the one you will have to use (will you focus on RNA Seq, resequencing, SNP detection?). Then download the data they used and try to handle it with common tools such as Bowtie, Cufflinks, TopHat, Crossbow,... depending on your need. You will have a first glimpse at the kind of data (and problems!) you will have to deal with. Then the course you will be attending will help you in a more efficient way I guess.

If you have a peculiar focus with this data maybe we can help you finding some litterature references and/or some convenient tools. There is also many review being written about NGS now. With the good keywords you should find some of interest for you on pubmed.