Hi,
Let's say that I get whole-exome-sequencing data file that has been created without the availability of a normal sample related to the tumor sample sequenced. Is there a way to make the disctinction bewteen germline and somatic variants. I was thinking of comparing the variants against the COSMIC (Catalog Of Somatic Mutations In Cancer) database.
So I was wondering if some people have some suggestions of a nice accurate workflow with other sources than COSMIC.
Thanks,
Fred
A small addition to point number 1: You could also look for known variants in the 1000 genomes project data.
Thanks a lot for sharing your experience. Really appreciate. Fred