Insilico Mutation Analysis For Amino Acid Residues

Question

[GR]

Hi All,

I want to mutate few selected amino acid residues in protein structure and want to insilico predict their affect on protein structure. Can anyone give me some guidelines to do this. Is it fairly simple to do this or requires lot of time and skills. I am completely naive to sturctural bioinformatics and it is not my area. But this small module of my project brought me in this.

Can someone help me Please.

Thanks, R.

Answer 1

[Vladimir Chupakhin]

There are many tools, take a look for example at

• I-Mutant 2.0.6 – A Tool for Predicting Protein Stability upon
• Mutation CUPSAT – Protein Stability Analysis Tool SNAP – Predicts
• Effect of Mutations on Protein Function iStable: off-the-shelf predictor integration for predicting protein stability changes.

Take a look also at Google Scholar for prediction + protein + mutation keywords. Here are example articles

• Prediction of protein stability changes for single-site mutations using support vector machines
• Large-scale prediction of protein geometry and stability changes for arbitrary single point mutations