How To Find A Read Spanning A Large Deletion In Bam?
1
0
Entering edit mode
11.6 years ago
siyu ▴ 150

How to find a read spanning a large deletion ,such as 1kb, in BAM? can anyone give me some advise ? Thank you very much !

read bam • 3.4k views
ADD COMMENT
1
Entering edit mode
11.6 years ago

No aligner (at least the ones I know) generates a bam (alignment) file that will give the straightforward information especially one you are looking for above.
Normally, the tools that are used to call for long Indels and other SVs using a BAM file will also generate an output file containing information baout which reads were used to detect these Indels or SVs. Pindel, breakdancer etc. are some of the example.

If you are looking for reads spanning a 1 kb deletion, no aligner will allow this big gap for the alignment so that you can directly extract the reads from the bam file. The other option would be to look for reads that are soft clipped and if you find many reads that are aligned as soft clipped around a given region (breakpoint) then it can help. Other, strategy would be find the pair of discordant reads that have been aligned around the indel with insert size less than the actual one but these reads will not be spanning the Indels.

ADD COMMENT
0
Entering edit mode

Thanks for your answer ! "no aligner will allow this big gap for the alignment so that you can directly extract the reads from the bam file",how to extract the reads from the bam ? would you give me a detailed description? I have no idea about it ?

ADD REPLY

Login before adding your answer.

Traffic: 1867 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6