Question

How To Know Which Is The Start Codon And Stop Codon For A Gene Sequence?

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13.7 years ago

Elena ▴ 250

Hi Friends,

I have a sequence and want to know the start site and the stop site in that particular gene. for eg: NM_000465 is my gene sequence and want to know the start site and the stop site of the sequence so that i can translate the sequence to a proper protein sequence.

genome sequence position • 36k views

ADD COMMENT • link updated 13.7 years ago by Neilfws 49k • written 13.7 years ago by Elena ▴ 250

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In the case of completely annotated sequences, search NCBI Nucleotide for your sequence and check the link for the protein record. If it was not available, you could use tools like ORF Finder: http://www.ncbi.nlm.nih.gov/projects/gorf/

ADD REPLY • link 13.7 years ago by Michael Schubert ★ 7.1k

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And don't use ALL CAPS!

ADD REPLY • link 13.7 years ago by Michael 55k

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@Schubert: Thanku & @Dondrup:okay

ADD REPLY • link 13.7 years ago by Elena ▴ 250

score 4 · Answer 1 · 2011-03-31

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13.7 years ago

Neilfws 49k

There are lots of ways to address this but figuring out the start, stop and coding sequence from sequence alone is not the best of them.

Just go here, where you'll find that start codon = 139, last coding codon (before the stop) = 2469 and look at the CDS field for the protein sequence.

ADD COMMENT • link 13.7 years ago by Neilfws 49k