Question

Best Way To Visualize Next Generation Sequencing Tumor Evolution In A Graphic?

4

Entering edit mode

11.5 years ago

Wayne ★ 1.0k

I have a good deal of NGS data with Normal, tumor, and Relapse samples. I would like a way of graphically depicting evolution regarding different mutations and genetic events. I have data on point mutants, CNV, and fusions but no good way to represent it as an evolving tumor. Does anyone know of a figure, program, or style of graphic that would be great in vizually showing important genetic changes in tumor evolution across many samples? Any help greatly appreciated.

ngs evolution sequencing • 3.6k views

ADD COMMENT • link updated 11.5 years ago by Malachi Griffith 20k • written 11.5 years ago by Wayne ★ 1.0k

score 4 · Answer 1 · 2013-06-04

4

Entering edit mode

11.5 years ago

Malachi Griffith 20k

How about something like the depiction used in Figure 2 of this paper: Clonal evolution in relapsed acute myeloid leukaemia revealed by whole-genome sequencing

Ding et al. 2012

ADD COMMENT • link 11.5 years ago by Malachi Griffith 20k

0

Entering edit mode

This is beautiful.

ADD REPLY • link 11.5 years ago by Daniel ★ 4.0k

0

Entering edit mode

Hello,

Do you know with which Software they did this figure ?

Thank you

ADD REPLY • link 10.1 years ago by xenia • 0

score 1 · Answer 2 · 2013-06-04

1

Entering edit mode

11.5 years ago

JC 13k

Some previus examples only show key points suring tumor progression, such as http://www.pnas.org/content/105/11/4283/F5.large.jpg

In your case, maybe you can plot some karyograms showing coverages, duplications and marking all events as occured in time. Please share your plot ...

ADD COMMENT • link 11.5 years ago by JC 13k