Length Of The Vdj Segment In B Cells

Question

[ericmajinglong]

Hi guys,

I'm not sure whether this immunology question can get an answer here, but I was wondering what the length of the VDJ segment is in B cells? Is there any way to find this out bioinformatically?

Thanks a lot!

Cheers, Eric

[Michael]

Your question is a little abridged, I cannot even say if its off-topic or not ;) What is a VDJ segment? Most computational people might not know, please explain your acronyms: see How to ask Good Questions on Technical and Scientific Forums

[corn8bit]

Yes, or the asker can link to further information using markdown tags. [Like this]biostars.org)

A VDJ segment looks like it has to do with some random gene in every immune cell.

[Shicheng Guo]

Dr. Tipton mentioned some bioinformatics solutions to your question in his Nature immunology paper. You can find the pipeline like this way:

Answer 1

[Noel]

You will find all you need to know here (Not sure you meant length of V(D)J before or after recombination)

Answer 2

[mizraelson]

The length of the VDJ region depends on the :

• V and J genes as they have different length
• Number of added nucleotides in CDR3 region
• Heavy chain has D segment while Light doesnt

Usually the average CDR3 receptor length is calculated for the repertoire: https://docs.milaboratories.com/mixcr/reference/mixcr-postanalysis/#cdr3-metrics