Merging Illumina Paired End Reads

Question

[vijay]

Dear All,

I have fastq a dataset containing forward and reverse sequences obtained through paired end module of Illumina platform. I am trying to merge these paired end reads. I have a query which I would wish to get cleared before I proceed further. Do I need to get the reverse complement of the reverse sequence dataset in order to carry on with the paired end merging?

I have referred few papers and tutorials on this , but they have not mentioned anything about doing a reverse complement. I am bit confused in this step. Kindly help me out!!!

Responses are highly appreciated!!!

Answer 1

[Pierre Lindenbaum]

duplicate of:

merge paired-end reads

Answer 2

[Buttonwood]

Do you mean overlap merge? Both of the reads are from 5'-->3'.