Entering edit mode
11.4 years ago
steve
▴
40
This question is a follow up from: Are some sequences uniquely mapped by BWA when mapped as paired-end that would not be when mapped as single-end?
My question is:
How does BWA-MEM report single-end mapping quality?
BWA-backtrack kindly reports the single-end mapping quality using the SM:i tag. But is there a way (hackish or otherwise) to get BWA-MEM to report this field? If not, why?
EDIT:
These tags would also be nice:
XT; XA; X0;
So for BWA-MEM, is
SM:i
always equal toMAPQ
? Is that why Heng removed this from the output? IF that's the case I can just duplicate the field. easy.these are slightly different meanings of mapping qualities some discussion is here
Differing BWA MAPQ scores for a read depending on how I build the transcriptome
the way mapping qualities are obtained are somewhat mysterious and perhaps it is better there is only one of them
Any thoughts on how MAPQ is assigned?