What Is The Difference Between Rna-Seq And Transcriptome Sequencing?

Question

[toshnam]

Hi all,

I guess they are different.
Are they different?
If so, what is the difference?

Thanks in advance.

Answer 1

[David Quigley]

I think these are synonyms. RNA-seq is a specific means to measure the state of the transcriptome.

[Chris Evelo]

+1 Nice link, and it is always tricky to stick your head out by questions where there might be some semantic nuance that you were not aware of.

Answer 2

[Chris Evelo]

There is a very nice [?]description in Wikipedia[?]. There are a few different technologies and approaches but, like David said, the two terms are indeed synonyms as long as you mean whole transcriptome sequencing.

Answer 3

[Alex]

I think RNA-Seq is more wide term. RNA-Seq includes transcriptome sequencing (polyadenylated RNA), "all" RNA sequencing (include rRNA and non-coding RNA) and variant for various non-coding small RNA sequencing. The main difference in a method of probe preparation.