Hmmalign Bit Scores
1
0
Entering edit mode
11.2 years ago
Maria ▴ 170

Hello, I recently start using hmmer 3 more specifically hmmalign in hmmer it takes an hmm model already build by hmmbuild and a fasta file containing multiple sequences. Previously I used infernal for align rna (cmmalign) . I used to take the total bit scores to do some analysis. The question are there any bit score in the output of hmmer ? if not is the bit score for any hmmer version available ? and which one ?

alignment sequence model hmmer • 4.4k views
ADD COMMENT
2
Entering edit mode

As far as I can tell 'hmmalign' does not output any scores, however you may find that the contacting the HMMER developers (hmmer@janelia.hhmi.org) helpful, since they will be able to tell you if there is a way of obtaining scores or if they plan adding scores in future.

ADD REPLY
2
Entering edit mode
11.2 years ago

You'll find a lot of useful information in their userguide ftp://selab.janelia.org/pub/software/hmmer/CURRENT/Userguide.pdf especially from page 37 to 40 where tabular output formats are described. Several bit scores are available.

Moreover, something good to know is, while building your own HMM, you can compute some cutoffs (e.g. "trusted cutoff") taking into account some min or max scores between your model and the sequences used to make it. If you have this information in your hmm, hmmscan or hmmsearch are able to filter hits according to it.

ADD COMMENT
1
Entering edit mode

Thanks for upvotes, but I got mixed up with hmmscan. I don't think you have a bit score with hmmalign...

ADD REPLY

Login before adding your answer.

Traffic: 2051 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6