Illumina Read Quality

Question

[hellbio]

Hi all,

I am new to NGS and i have a very naive question.

Illumina reports the score of each base in the read as a phred score. But, how is the total read quality calculated?Is it the sum of scores of all the bases in the read? Any comments are valuable.

[always_learning]

Similar one was discussed here

Answer 1

[Josh Herr]

There are a lot questions here focused on read quality so your question is redundant -- you can find other questions relating to your topic by using the custom search.

I would also recommend this page -- FASTQ format and read quality -- to provide you some background on how the Phred quality score is calculated and what each character means.