Definiton Of Singletons

Question

[mujupas]

Hi all,

I wonder if anyone would care to tell me what is the official definition of reads falling in the "SINGLETONS" category according to Flagstat (in paired end sequencing)

Thank you in advance for your kindness! gio

Answer 1

[Sam]

You can find a relevant discussion here

Basically, it should be reads that only one of the pairs are aligned to the genome

[mujupas]

Mmm thanks but why the "should be"? Isn't there an official agreement about this? It's actually surprising that it's not so easy to retrieve this information...