How To Calculate What Gene Expression Is Significant In Cuffdiff

Question

[newDNASeqer]

I have three sample columns in cuffdiff output, and I know the last column in the output result gene_exp.diff indicates whether the gene expression is significant. But I need to do some statistical work myself. Based on the three fpkm values, is there a way to calculate whether they are significant?

[Phil S.]

Do you mean whether one gene is differentially expressed between your three samples?