Does The Reading Frame Of The Exon Always Start At The First Nucleotide

Question

[davidvanijzendoorn]

I have the sequence from an exon. Does the reading frame always start at the first nucleotide of the exon for when I want to translate it to an amino acid?

Answer 1

[swbarnes2]

No, it doesn't. There's no way to know just by looking at an exon what frame is the right frame for translation.

[Phil S.]

If you know the length of your 5'UTR you can calculate the frame from the exon sequence, right?

[swbarnes2]

If you know what position that exon sits in the whole transcript, then sure. But you can't know that from the sequence of an exon alone.