Mirna Expression Differences Using Mirdeep2

Question

[kmherb]

I am using mirDeep2 to look at expression differences across samples. I think mirDeep2 normalizes the samples versus total number of miRNA reads. Is this correct? I might expect differences in the number of total number of miRNAs produced from one sample to another, since I am hitting a biogenesis factor. Is there a better way to normalize my data to account for actual differences in the total population of expressed miRNAs?

[chentong.biology]

mirDeep2 normalizes miRNA expression versus total number of miRNA reads.

You may try normalizing the read count of each data set to that data set’s count of tRNA-, snRNA-, scRNA-, and srpRNA-derived reads; as mentioned in article.