Which Program Do You Use For Converting Srf (Short Read Format) To Fastq?
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13.5 years ago

I am looking for one (or several) ways of converting .srf files to fastq. Which would be the best program to use? if you know several of them, would you recommend one over the rest, and why?

Related with that, does any of the popular (bwa, bowtie, novoalign etc) aligners takes .srf files as input?

next-gen sequencing fastq • 3.8k views
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13.5 years ago
Hranjeev ★ 1.5k

The Staden Package and its IO library is a popular choice to convert reads to and fro different formats. It supports srf to fastq conversion from what I know.

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13.5 years ago
Pasta ★ 1.3k

I second HRanjeev answer. Use Staden Package with :

srf2fastq -c file.srf > file.fastq

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