Why 3' End Has A Lower Quality In Ngs Data

Question

[Kash]

Hi all,

I observed that NGS sequence reads have a lower quality towards the 3' end. I would like to know the reason for having low quality reads towards 3' end. Hope someone can help me.

Thank you inadvance

Answer 1

[Ashutosh Pandey]

Check this post on a different forum

[Kash]

Sorry to bother you again but I can not access this link. Can you please copy the content of thread here

[Devon Ryan]

I've updated ashutoshmits's answer with the correct link. The gist is that there is an accumulation of errors and noise over time (the same phenomenon occurs in sanger sequencing).

[Kash]

Thank you very much. I got the point