Entering edit mode
10.9 years ago
Anima Mundi
★
2.9k
Hello everybody, I am trying to BLAST a nucleotide query against a nucleotide database via command line.
The problem is that, when I try blastall -p blastn -d mydatabase -i myquery
I get just:
BLASTN 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= myquery
(52 letters)
Nothing else is displayed, even when trying to make positive control experiments. What is wrong?
Thanks in advance.
Have you tried blasting your sequences on ncbi server? Just to check if they are ok.
I just tried, it works on NCBI.
Consider this answer - A: Help with formatdb and blast all (
blastall
vsblastn
)Thanks; actually, I was trying to use blastall to avoid a problem in the format of my subject database while using blastn. Maybe I will open a new question to fix that issue.
Here is the new question: Database error when running blastn