How To Go From Ncbi Blast Results To Go Term Id'S Fo Gene Ontology

Question

[Samantha]

I have currently completed the blasting and annotation of a large data file consisting of de novo aligned Illumina sequencing reads. However the annotation file only gives me the gene name, the p-value, the contig number, and the NCBI Accession ID. The organism is a freshwater mussel, so not a commonly annotated organism.

My question is if there is a converter that can take those accession ID's and change them to GO ID's so I can create Gene Ontology figures to summarize the various biological processes these genes represent. I have tried various programs like DAVID and BIOMART but they seem to require that my gene accession list be of one specific type or I have to choose a model organism to convert with. I am getting errors left and right.

Am I missing something simple? Any help would be appreciated.

[upendrakumar.devisetty]

You can directly upload the .annot file onto blast2go suite (gui) and there are options to make summary figures. You can also convert your .annot file to wego format and then upload your wego formatted file into http://wego.genomics.org.cn to generate figure related to classification of transcripts into different GO functional categories. Hope this helps.....

Thanks Upendra

Answer 1

[chefer]

You can try to use Blast2GO to annotate your gene list with GO terms. If I remember correctly you can upload a xml file with your blast results and it will perform GO annotations on your dataset.