What is the state-of-the-art tool for miRNA target prediction? I have a FASTA file with sequences and would like to predict miRNA targets in these sequences. Thanks, Gregor
What is the state-of-the-art tool for miRNA target prediction? I have a FASTA file with sequences and would like to predict miRNA targets in these sequences. Thanks, Gregor
Different miRNA target prediction programs produce different results and have high false positive rates. You can use CLIP-Seq datasets to further filter the predicted miRNA targets. starBase collect and curate 111 CLIP-Seq (PAR-CLIP, CLASH, HITS-CLIP, ICLIP) datasets.
You can download them at the download page: http://starbase.sysu.edu.cn/download.php
You didn't mentioned for plants or animals. You can try:
sPARTA for plants - http://nar.oxfordjournals.org/content/42/18/e139
Download Link for sPARTA: https://github.com/atulkakrana/sPARTA.github
miRZA for animals - http://www.nature.com/nmeth/journal/v10/n3/full/nmeth.2341.html
miRZA download requires registration, follow the paper
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Miranda would be of great use.