Some Non-Variant Positions Obtained When Using Bcftools With -V Option. Any Idea Why This Happen?
0
0
Entering edit mode
10.7 years ago
dapregi ▴ 50

Hello, I am wondering why, if I have used the -v option in bcftools (output only variant sites), I have in the output some reference homozygotes. Could it be because the genotype likelihood is too low that could be also a het? Does the -v option look at the most probable genotype (the one written down) or at the genotype likelihoods?

bcftools view -Svc myfile.vcf.gz

#CHROM  POS     ID      REF     ALT     QUAL    FILTER  INFO    FORMAT  MET_03912_4_1_TAGCTT
[...]
1       15906   .       A       .       3.55    .       DP=3;QS=0.000000,0.906977,0.093023,0.000000;VDB=4.034274e-02;AF1=1;AC1=2;DP4=0,0,0,3;MQ=16;FQ=-33;AF1=1;AC1=2;FQ=-30    PL:DP   31,3,0:3
vcf vcftools bcftools • 2.4k views
ADD COMMENT
0
Entering edit mode

Heads up: This is a 4 year old post.

ADD REPLY

Login before adding your answer.

Traffic: 2521 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6