Sequence Depth Genome

Question

[Oduro]

how is average sequence depth calculated from number of reads and read length

Answer 1

[Pasta]

I understand this concept as equivalent to coverage.

On a genome it means that each base has been sequenced a number of time.

Coverage = (Nb of Reads)*(Read Length) / (Genome Size)

[Chris Evelo]

I think the second line should read:

On a genome it means that each base has on average been sequenced a number of time.

Answer 2

[Boboppie]

Relevant questions are:

• What is the sequencing ‘depth’
• What is the difference between sequencing depth and coverage

@Istvan Albert pointed to the "Coverage" section of "Shotgun sequencing" on Wiki, same as @pasta.