Per base sequence content of FastQC Report

Question

[yueli7]

Hello,

I have result of RNA sequencing FastQC Report.

It was failed in Per base sequence content, but passed Adapter Content.

I went to https://sequencing.qcfail.com/articles/positional-sequence-bias-in-random-primed-libraries/.

It seems the primers problems.

How I can fix this?

Thanks in advance for great help!

Best,

Yue

Answer 1

[GenoMax]

Appears that my answer from yesterday was lost during the cut-over to the new biostars codebase. Basically, there is nothing to fix here as the linked blog post concludes. The data should align well.

[yueli7]

Hello GenoMax,

Thank you so much for your great again!

Thank you!

Best,

Yue

Answer 2

[JC]

it could not be a problem, the recent library preparation uses a transposase system that generates that pattern on the first bases, check what kit was used.

[yueli7]

Hello JC,

Thank you so much for your great help!

Thank you again!

Best,

Yue