How to get started with transcriptome sequencing as a bioinformatics beginner.

Question

[mariam28071993]

Hello,

I am just starting to learn bioinformatics to be able to analyze my RNA-seq data (from whole transcriptome sequencing). Is there any advice on where to start, because I am feeling lost?

Thanks

Mariam

Answer 1

[rpolicastro]

Bioconductor has a great tutorial that walks through the whole process. You can find it here. Also refer to the DESeq2 and edgeR vignettes.

[mariam28071993]

@replicocastro Thank you so much!

Answer 2

[GenoMax]

Do you have experience with linux comand line? If you don't that may be where you may want to start. Here is a simple tutorial for that: http://korflab.ucdavis.edu/unix_and_perl/current.html#part1

If you are not able to use the command line then using online Galaxy may be an option: https://galaxyproject.github.io/training-material/topics/transcriptomics/tutorials/ref-based/tutorial.html

If you are willing/able to spend some money then Biostar handbook may be an option to look at.

[mariam28071993]

@GenoMax Thank you so much for the links. No, I don't have experience with Linux.