How to find the true orthologs from recipocal blast hits (blastn) ?

Question

[sunnykevin97]

Hi,

After recipocal blast (blastn) analysis, from 7 different species, I got a ~10,000 orthologs as a final set. How do I find the true orthologs from the final set ?

Suggestions please!

Thanks

[Juke34]

Without phylogeny analysis there is a high error rate, but using MCL like OrthoMCL could probably help.

[Asaf]

Have you tried using databases of orthologous proteins like COG or EggNOG? The problem of assigning orthology groups is not simple at all.

[Juke34]

Have you look at OrthoFinder2?

[sunnykevin97]

Yes, I already did the analysis. Orthogroups_for_concatenated_alignment.txt file shows 1088 sequences. Can I consider these as orthologs ?

Or else, which folder/files do I consider the orthologs sequences ?

Thanks Kevin.