Entering edit mode
3.6 years ago
Lila M
★
1.3k
Hi there,
I'm trying to plot a metagene profile for a set of peaks. So far, I'm using makeMetaGeneProfile.pl
(homer software) as follow:
makeMetaGeneProfile.pl rna hg38 -size 3000 -hist 10 -p mypeaks > metagene_profile_peaks_
and my peaks looks like this:
peaks_41 chr1 2124076 2124431 *
peaks_43 chr1 2155055 2155929 *
peaks_81 chr1 3605028 3605680 *
peaks_171 chr1 7147516 7147860 *
apparently the program works as it doesn't give me any error and generates the output, the problem is that all the values are zero, which makes me things something is going wrong.... any advice or idea? Maybe there is another software that I can use with bed files
PS. I've used computeMatrix
from deeptools
, which work incredible well if you have original bam files, but for this approach, I do not feel very confident neither.