bedGraphToBigWig

Question

[mropri]

I am doing some ChIP seq analysis and was trying to convert my bedgraph file to bigwig using bedGraphToBigWig package so I could visualize my peaks in the Genome browser. However, when I run the bedGraphToBigWig command, I get the following error:

bedGraphToBigWig: error while loading shared libraries: libssl.so.1.0.0: cannot open shared object file: No such file or directory

I am confused because I have anaconda installed in the same directory that I am running the command from. Any help is appreciated. Thanks

Answer 1

[jared.andrews07]

Did you install bedGraphToBigWig utility with conda as well? If not, this should fix it:

sudo apt-get update
sudo apt-get install libssl1.0.0 libssl-dev

If you did install via conda, did you install into base or an environment you created?

[mropri]

I installed it to the base environment. Also, I dont have permission on the university cluster I am running the command on to run sudo. Is there another way to go about this or is that it?

[boczniak767]

Then the only option is to ask the system administrator to install it for you.

[jared.andrews07]

How did you install it? Via conda? It should really take care of any dependencies, since libssl is available via conda as well.

I would make a fresh environment and try again, installing into the base environment is generally ill-advised.