Make info tag separate column VCF
0
0
Entering edit mode
3.6 years ago

I am running ANGSD to get theta values for a VCF (I need to use a software that can account for small sample size/lower coverage). I have a PL (which I understand to be the same as GL) tag in my info field and PL (via GATK) values for my SNPS. However, when I run ANGSD I get the message

BAD SITE scaffoldx:y. return code:-2 while fetching GL tag rec->rid:3

giving me the impression it is not accessing my PL values. I changed my PL tags to GL, in my vcf and the error persisted. The output from ANGSD also states:

will use chrom, pos PL columns

This gave me the impression that maybe I need to make PL as a separate field rather than keeping it as one of my INFO tags. Is there a way to change a PL INFO tag to a separate field?

Thanks

vcf ANGSD • 946 views
ADD COMMENT
0
Entering edit mode

Please include the angsd command that you're using. You may want to email the authors of ANGSD with a link to this post so they can help you - it is not a popular software on the forum.

ADD REPLY
0
Entering edit mode

The command I used was

/angsd -vcf-gl angsd_x.vcf -doMajorMinor 1 -domaf 1
ADD REPLY

Login before adding your answer.

Traffic: 1766 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6