Entering edit mode
3.6 years ago
simplitia
▴
130
Hi I have a file with list of read ids I would like to extract from a pair-end read. This file only contains 1000 reads. When i use bbmap to accomplish this with the following command however, the resulting size of the fastq files are very similar to original and so are
/bbmap/filterbyname.sh in1=${fq1} in2=${fq2} out1=strip2_R1.fq.gz out2=strip2_R2.fq.gz names=names2.txt
This should only have 1000 reads in the new files but its in the millions!
Is there something I'm doing incorrectly? thanks!
What does
names2.txt
look like (show us output ofhead -5 names2.txt
)? Remember to remove@
or>
identifiers from read names innames2.txt
. One read name per line in file.Hi, ok here is how the first few lines look like.
You may need to add
substring=t
. These should work.