Combining distance matricies between multiple aligned genes into final distance matrix

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3.5 years ago

robert.murphy ▴ 90

I have alignments of Busco genes present across all my assemblies. I want to get a final distance matrix based on these individual gene alignments so I can plot an MDS.

I plan to generate either a similarity matrix with Rs Seqinr package or a distance matrix with RaxML for each gene. Firstly are similarity and distance here just the same with reversed given that the distances are euclidean I believe?

Secondly, how can I combines all these matrices into a single one?

phylogeny distance alignment • 965 views

ADD COMMENT • link 3.5 years ago by robert.murphy ▴ 90

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Wouldn't it make more sense to concatenate all alignments first and then proceed with your phylogenetic analysis based on a single MSA? Gene-wise calculation of distances gets rid of length information of different gene-based alignments unless you somehow bring them back into the calculus.

ADD REPLY • link 3.5 years ago by Michael 55k

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I am not sure I understand what you mean. I am taking all (for example) BUSCO 929at5338 genes across all 46 samples and performing an MSA. I am unsure how I could concatenate those alignments with all others into one and and use it for distance analysis.

ADD REPLY • link 3.5 years ago by robert.murphy ▴ 90

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Could I not just multiple the distance value by gene length to weight every distance by thee length then sum all the dataframes together?

ADD REPLY • link 3.5 years ago by robert.murphy ▴ 90

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