Entering edit mode
3.5 years ago
zizigolu
★
4.3k
Hi
I have downloaded log2(TPM/10+1) values of 10X Malignant ascites single cell from GSE146026 as raw data is not available
Is there anyway to use this matrix and convert that to a Seurat object?
Thank you so much for any idea
Sorry you can help with this question please?
https://bioinformatics.stackexchange.com/questions/15948/making-seurat-object-from-a-processed-file
I want to make Seurat object having all of cell barcodes, genes, patients ID but I really can not find the answer
Read10X
reads 10x output folders, and it requires the content to be exactly what it is supposed to be output bycellranger
(i.e. the triad of files features, barcodes, matrix). If you are providing your own matrix you need toCreateSeuratObject(counts = yourMatrix)
. Just make sure that you have genes and cellsID asrownames
andcolnames
.