Read mapping statistics
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3.6 years ago
kathrynm • 0

Hello!

I am looking at the read mappings for my taxa and want to make sure I correctly understand what each column represents.

In rPHG, what are the taxa_count and range_count columns generated from readMappingsForLineName?

In the output txt file for each taxon ending with _additionalMappingStats, what are count, totalNM, totalAS, totalDE and listOfStartPos?

If there is already documentation on this that I missed, please let me know! Thanks! Kathryn

phg • 805 views
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3.5 years ago
zrm22 ▴ 40

For rPHG - range_count is the number of reads mapping to a given reference range. taxa_count is the number of those reads mapping to a specific taxon in the range.

For the _attionalMappingStats files, they are just counts of some of the mapping statistics coming from the BAM/SAM files. 'count' is the number of reads which hit a given haplotype. 'totalNM', 'totalAS' and 'totalDE' are the cumulative sum of the NM, AS and DE fields in the BAM/SAM file for a given haplotype.

Finally listOfStartPos are the alignment start positions for each read with respect to the haplotype. Basically it allows you to look at how the reads are distributed along the sequence of the haplotype.

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Thank you!

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