RNA-seq Normalisation methods to compare using raw miRNA data

Question

[raavi21198]

Hello members!! I have a raw data file of miRNA sequence data (Read counts file). I need suggestion for few normalisation methods that I could use to normalise the data. So far i have used (Size factor and logFC paramaters of) DESeq2 and (TMM of) EdgeR. I cannot use RPKM because the raw data file doesnt contain any read lengths. Kindly advice me what can be considered for the same Thank you

[Gjain]

rlog or vst worked nicely for me but you have to check with your data what works best for you. Make and RLE plot and see the differences.

[raavi21198]

Sure. Thank you for your response!!