Question

Finding adapters TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold

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4.0 years ago

newb • 0

Hi,

I have some fastq files and I want to remove adapters. The kit used was "TruSeq Stranded Total RNA with Ribo-Zero - H/M/R_Gold" but I can't find any info on it. Anyone know where I can find the adapters used for this kit?

rna-seq fastq illumina • 2.0k views

ADD COMMENT • link updated 4.0 years ago by Istvan Albert 102k • written 4.0 years ago by newb • 0

score 2 · Accepted Answer · 2021-05-26

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4.0 years ago

GenoMax 151k

fastp will identify possible adapters in your data automatically.
You could use bbmerge method - How to figure out adapter sequence for Illumina reads?
These should be standard TruSeq adapters. You can find the sequence in Illumina's site here.

ADD COMMENT • link 4.0 years ago by GenoMax 151k

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Thanks a lot for your response!

There are several TruSeq kits on Illumina's site and they don't seem to all have the same kind of adapters. Do you know which kit I should look at?

ADD REPLY • link 4.0 years ago by newb • 0

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See https://support-docs.illumina.com/SHARE/AdapterSeq/illumina-adapter-sequences.pdf

TruSeq is always the same adapter sequence, starting with AGATCGGAAGAGC for both reads.