How to use transcript coverage for ORF calls on contig?
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3.5 years ago
O.rka ▴ 740

I have a metagenomics assembly and I've identified several contigs that are bona fide viral contigs (I used virsorter2 and checkv). I have paired metatranscriptomics data for these samples and I mapped to the viral contigs. I then converted those bam files to bed files using bedtools genomecov.

Is there any way I can use the coverage profiles with the contig assembly to get better ORF calls than prodigal?

gene-calling virus metatranscriptomics metagenomics • 572 views
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