Query regarding WGCNA module formation

Question

[microorganism_001]

I want to form a WGCNA module, I took the genes which are expressed highly according to their standard deviation of my desired sample group, I want to ask is their need to normalize the gene count series matrix again before doing the experiment?

Please tell me there is a total of 9 samples and I choose highly expressed 4000 genes in 9 samples. What type of normalized procedure is needed?

Answer 1

[andres.firrincieli]

Don't bother using WGCNA if you have just 9 samples. WGCNA authors suggests > 20 samples, while 15 is the minimum number of samples (link)