How To Resolve Discordance Between Mirna And Mrna Results Obtained With Genespring

Question

[Mandar]

I have analyased the miRNa and mRNA expression profiles of pancreatic cancer using the genespring software,But how I shall make sure that I have analyed it correctly because results vary among the differents softwares,diff parameters and algorithms used in whole analysis.Is there any way to check?

[Istvan Albert]

You need to be more specific than that.

What do you mean by discordance, what do you consider as being different and why should the results be the same

Answer 1

[pld]

You answered your own question. The results for a given data set vary between different analysis because you changed the analysis settings.

[Mandar]

So how to check that whether I have done the analysis correctly or not?

[pld]

I don't think you can break genespring, but check their documentation and with people who have done similar work that you are doing.