whole genome sequence for origami 2 (DE3) and rosetta 2 (DE3)

Question

[mittu1602]

Dear all, Looking for whole genome sequence of competent cells Origami2 (DE3) and Rosetta2 (DE3) from Novagen. any lead on how can I procure it?

Except mailing the company - already done, awaiting their response.

Thank you

[Sonia]

Hello, hope you are doing well, I am PhD student at Monash University Malaysia. Currently working on Rosetta-Gami 2(DE3). I am looking for Rosetta-Gami 2 (DE3) whole genome sequence for some cloning purpose. I saw your post asking for the same thing. If you got the sequence already please share with me. OR do you have any idea how can i get that, so, i'm looking for your guidance. I tried on NCBI nut no clue.

Answer 1

[Mensur Dlakic]

Not sure this is a bioinformatics question.

I doubt you will get any meaningful response from the company. These are derivatives of BL21 (DE3) cells, which starts with a BL21 strain:

• https://www.ncbi.nlm.nih.gov/assembly/GCF_013166975.1
• https://www.ncbi.nlm.nih.gov/assembly/GCF_014263375.1

That is a majority of the sequence. This strain has an integrated lambdaDE3 fragment for which it should not be difficult to find sequence. Finally, there is a chl-resistant plasmid that carries rare tRNAs which is probably described somewhere, though I doubt the company will furnish this information.

[mittu1602]

Thank You for your response, I had the same thought process.

[Raygozak]

This answer is like being asked for the human genome and being given the yeast genome in response.