I want to know how to use htseq-count data for detecting DEGs by DESeq2.
We can get gene expression count matrix by htseq-count, and also we can use this output in DESeq2. However, the last 4 rows of htseq-count matrix are not count data but information about counting result.
Do everyone remove last 4 rows by themselves in R ?
Or are there any functions in DESeq2 to pass the raw result of htseq-count ?
So, in conclusion, I want to know how to connect htseq-count to DESeq2. Thank you.
HI,
I have two separate matrix for normal and control. DeSeq2fromHTSeqcount , may i have to run separately for each matrix. I want GE.txt for further analysis. Please help me to input the data in Deseq2