Testing the net differences between 2 treatments, over the control

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3.8 years ago

haasroni • 0

Hello all,

I have protein data for 2 different treatments and 1 control (3 biological replicas each). I want to find the net differences between the 2 treatment conditions, reducing the control. I am using a T-test for that purpose.

How it would be best to do it? Thank you!

T-test • 869 views

ADD COMMENT • link 3.8 years ago by haasroni • 0

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Be sure to check the literature on standards for the analysis of this type of data. If this is some kind of mass spec or similar I would check whether limma is an option as it is almost certain that this has more power than a naive t-test. What kind of data is this, and how many measurements are in there? Like dozens, or thousands?

ADD REPLY • link 3.8 years ago by ATpoint 88k

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Thank you for the help! I will look into the limma option. It is free DDA LC-MS data. I have a few thousand measurements.

ADD REPLY • link 3.8 years ago by haasroni • 0

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