Creating Signature Matrix from SC-RNA Seq data for Deconvolution of Bulk Tissue

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3.3 years ago

deontaepharr ▴ 10

Hey,

I've been trying to understand how to create a signature matrix but there's no tutorials to follow and the papers I've found from Cibersort and others lack in detailing step by step. So, can anyone provide some insights on the matter. One paper that I have followed intimately has this section (image attached below) but the resulting matrix doesn't work in downstream processing.

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deconvolution rna-seq cibersort • 2.1k views

ADD COMMENT • link updated 3.3 years ago by Evan ▴ 250 • written 3.3 years ago by deontaepharr ▴ 10

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Hello, Cibersortx website has a nice tutorial about it. To clarify, I suggest you to go on this thread I posted few months ago to know more : [SOLVED] How to build a table with gene expression per cell type with Seurat ?

ADD REPLY • link 3.3 years ago by Evan ▴ 250

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