Entering edit mode
3.4 years ago
Analeigh
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0
Hi! I have been reading the BD single-cell multiomics bioinformatics handbook (https://scomix.bd.com/hc/en-us/articles/360019763251-Bioinformatics-Guides).
For its targeted assays (an assay with 399 immune-related genes), there is an option to "map all of the reads only to the forward strand of the provided reference".
I was wondering under which circumstance that I need to do this. To speed up alignment?
But genes are roughly 1:1 on both reverse and forward strands of the reference, wouldn't this let me lose information?
Have you looked at the schematics of the kit? Perhaps they are only capturing one specific strand?