Remove PolyA 3'End with cutadapt

Question

[GiV17]

Hi all, How Can I remove the polyA tails and the adapter sequences of my fastq file using cutadapt software? Has anyone tried it already? Thanks

Answer 1

[ATpoint]

Unsurprisingly the manual contains the answer in the FAQs:

https://cutadapt.readthedocs.io/en/stable/recipes.html#trim-poly-a-tails

[GiV17]

oh Yes, Thanks, But I would like a feedback with those who have already used it. And also I would like to understand which is the best polyA window to fling 10? 100?